Gel Range — approximate DNA concentration

GelJockey

Drop a gel image, mark the gel and the ladder, get an approximate ng/µL range per sample.

Gel image

Drop a gel image here

or choose a file

JPEG / PNG work anywhere. 16-bit TIFF gives the best result (needs internet to load the decoder). DNG isn’t supported — export a TIFF or JPEG.

Open image

sample band ladder / reference crop region Tip: drag on the image to crop to just the gel. Click a lane to mark/unmark it as the ladder.

Settings

Signal channel

Reference band mass (ng)

Sample load (µL)

Range factor (±×)

Ladder = lane with ≥ N bands

Lane spacing (px)

0 = auto-detect the pitch auto

Sample estimates

Open an image to begin.

DNA Gel — Approximate Concentration Report

Sample	Estimate	Range

Method: band fluorescence intensity is integrated with local background subtraction and scaled to mass using one bright reference band of known quantity as the anchor. Values are order-of-magnitude estimates intended only to flag samples that may fall outside a normal input range; read the range, not the central figure. Not a certified concentration. For a tighter number, run a dilution series of the amplicon as the standard.